Cutting Edge: Ig H Chains Are Sufficient to Determine Most B Cell Clonal Relationships

Clonal relationships in recurrent B-cell lymphomas

Immunoglobulin (Ig) gene rearrangements remain largely unmodified during the clonal expansion of neoplastic cells. We investigated the clonal relationships between lymphoma components at diagnosis and at relapse by analyzing Ig gene rearrangements. A BIOMED-2 multiplex polymerase chain reaction (PCR) assay was performed in 27 patients using formalin-fixed paraffin embedded tissues, with subsequ...

Degradation of Unassembled Ig Light Chains Cutting Edge: Proteasome Involvement in the

The Immunoglobulin ( Ig ) a and Ig b Cytoplasmic Domains Are Independently Sufficient to Signal B Cell Maturation and Activation in Transgenic Mice

The B cell antigen receptor, composed of membrane immunoglobulin (Ig) sheathed by the Ig a /Ig b heterodimer plays a critical role in mediating B cell development and responses to antigen. The cytoplasmic tails of Ig a and Ig b differ substantially but have been well conserved in evolution. Transfection experiments have revealed that, while these tails share an esssential tyrosine-based activat...

Cutting edge: CTNNBL1 is dispensable for Ig class switch recombination.

Ig class switch recombination (CSR) and somatic hypermutation require activation-induced cytidine deaminase (AID). The search for AID-interaction factors has been a major research effort in the field, as the mechanism of preferential targeting of AID to Ig loci remains elusive. CTNNBL1 is one of the few identified AID-interacting factors and has been shown to affect AID-mediated mutation and ge...

Cutting Edge: Macrophages Are Required